Enhancing Interferometry with Dolomite’s Mitos Dropix

Researchers at Vanderbilt University have developed a novel interferometry approach – the compensated interferometry reader (CIR) – and are using Dolomite’s Mitos Dropix to provide reproducible droplet production. Interferometers are widely used in science and industry for the measurement of small displacements, refractive index (RI) changes and surface irregularities. Professor Darryl J. Bornhop and his group are using this patenteCIR technique in combination with label-free, free-solution assay to measure a wide range of biomolecules and biomolecular interactions. Professor Bornhop explained: “The key difference between our approach and other interferometry methods is that we haveliminated the need for high resolution temperature control. Based on a laser, a microfluidic chip, a capillary and a CCD array, CIR enables simultaneous differential RI measurements within nanoliter volumes. By using Dolomite’s Mitos Dropix microfluidic droplet generator, we can separate two solutions for simultaneous evaluation, allowing us tdiscriminate between a reference and a sample to offer detection limits down to 10-7 RIU, without the added complexity of a temperature controller.”

“We initially developed the CIR concept using our own, in-house microfluidic set-up, but were hindered by poor reproducibility for droplet generation. After probably a year of trying to build our own droplet generators, we heard about the Mitos Dropix, and realisethat this could be just what we needed. I called Dolomite, and we got one of the first of the instruments built, becoming a beta test site. Combined with the compensated interferometric approach – looking at two windows in one capillary with a single bea– this took our sampling speed and performance to the next level.”

“What makes this technique so unique is that it’s almost universal in natureit's compatible with antibodies and antigens, DNA and RNA, small molecules, ions and proteins. We have already used the system to explore a range of biomarkers in serum and urine, and we are now working to improve the robustness and user-friendliness of the platform for applications in both drug development and clinical diagnostics.”

Nice Insight

Nice Insight, established in 2010, is the research division of That’s Nice, A Science Agency, providing data and analysis from proprietary annual surveys, custom primary qualitative and quantitative research as well as extensive secondary research. Current annual surveys include The Nice Insight Contract Development & Manufacturing (CDMO/CMO), Survey The Nice Insight Contract Research - Preclinical and Clinical (CRO) Survey, The Nice Insight Pharmaceutical Equipment Survey, and The Nice Insight Pharmaceutical Excipients Survey.

Dolomite Microfluidics

Established in 2005, Dolomite Microfluidics has grown to be the world leader in the design and manufacture of high quality innovative microfluidic products. The company offers a range of microfluidic systems, components and specialist chemicals – including pumps, chips, connectors, temperature controllers, sensors, accessories and custom-made components – as well as software for analysis or automation. Modularity, ease of use, innovation and scalability are common to all Dolomite Microfluidics products, which are used across a broad range of applications in biology, drug discovery, chemistry, food, cosmetics and academia. Dolomite is a part of the Blacktrace group of companies, a world leader in Productizing Science®, and has offices in the USA, Japan, India, Brazil and Hanoi as well as a worldwide network of distributors.